Research
We develop highly multiplexed CRISPR/Cas-based technologies for precision genome engineering in microbes.
We BUILD scalable genome-editing technologies
We aim to UNDERSTAND the link between genotype to phenotype
We ENGINEER better biological systems and better editing tools
Build
Scalable genome editing technologies
We develop CRISPR-based technologies for precise genome engineering across diverse microbial systems. Our work ranges from establishing and optimizing genome editing in new organisms to developing approaches that enable precise genetic perturbation at scale. By combining genome editing with DNA barcoding and high-throughput screening, we can engineer and track thousands of defined variants in parallel. Building on approaches such as MAGESTIC, we are expanding these capabilities across microbial species and developing new ways to perturb both genomes and transcriptomes.
Understand
From genotype to phenotype
What makes one genetic variant matter while another has little effect? We use large-scale perturbation experiments to connect precise changes in DNA sequence to their functional consequences. We are particularly interested in synonymous variants and genetic interactions, uncovering how combinations of sequence changes ultimately shape molecular and cellular phenotypes.
Engineer
Better biological systems and better editing tools
We use genome-scale engineering to improve both biological function and the technologies that let us manipulate it. In microbes, we generate and screen large libraries of variants to optimize useful traits. In parallel, we apply directed evolution to CRISPR-associated enzymes, exploring sequence space to improve properties such as activity, specificity and performance in different biological contexts.